Open Source 3-D Printed ISO 8655 Compliant Multichannel Pipette
FA info icon.svg Angle down icon.svg Source data
Type Paper

Cite as Citation reference for the source document. Chinchane, S., Kadam, H., Mowade, K., & Pearce, JM. 2022. Open Source 3D Printed ISO 8655 Compliant Multichannel Pipette. Journal of Open Hardware, 6(1): 1,

pp. 1–18. DOI: https://doi.org/10.5334/joh.36 Academia.edu
FA info icon.svg Angle down icon.svg Project data
Authors S. Chinchane
H. Kadam
K. Mowade
Joshua M. Pearce
Location London, ON, Canada
Status Designed
Modelled
Prototyped
Verified
Verified by FAST
Uses 3D Printing
Links https://osf.io/9tn6e/ https://doi.org/10.5334/joh.36
OKH Manifest Download
FA info icon.svg Angle down icon.svg Device data
Assembly instructions https://doi.org/10.5334/joh.36
Design files https://osf.io/9tn6e/
Hardware license CERN-OHL-S
Certifications Start OSHWA certification

Multichannel pipettes are used widely to accelerate research and testing in life sciences laboratories and within the biomedical industry. Commercial multichannel pipettes cost several hundred to thousand U.S. dollars and are not accessible in many areas. This study utilizes an open source and digital distributed manufacturing model to design a USD$24 4-channel 200 micro-liter pipette. The design uses widely-available interchangeable off-the-shelf parts and custom components, which can be fabricated with a low-cost fused-filament RepRap-class desktop 3D printer. The manuscript describes the design, construction, and validation of an ergonomic open-source multichannel pipette, as well as the evaluation of pipetting parameters and quality control. The open source multichannel pipette is found to be compliant with ISO 8655 standards for precision and accuracy. The device has separate syringes that operate individual tips, which prevents cross contamination of specimens because there is no direct contact of different liquids with each other and no mixing with actuating air. Each syringe is easily replaced for different experiments and the source CAD files are provided to enable others to build variants or custom configurations of the open source multichannel pipette.

Buildmultipip.png

The assembly process of the open source multichannel pipette: a) the syringes being assembled individually with springs, tips and tip holders and being inserted into the syringe holder, b) twisting motion needed to lock syringes into syringe holder, c) M3 nut and bolt are inserted into the enclosure, d) the main plunger in inserted into the enclosure, e) enclosure is tilted up so that the sliding surface that will hold the syringe holder is exposed, f) the main plunger is pushed up into the enclosure as far as possible and the syringe holder is slid into the enclosure by depressing the syringes against the springs, g) the syringe holder is pushed to the end of the slide in the enclosure, and h) the remaining enclosure component is slid into place.

  • Main design files: https://osf.io/9tn6e/
  • Target group: chemistry, biochemistry, biology, biomedical, medical, and associated disciplines
  • Skills required: desktop 3D printing – easy, mechanical assembly – easy

See also[edit | edit source]

FA info icon.svg Angle down icon.svg Page data
Keywords open source hardware, 3d printing, additive manufacturing, distributed manufacturing, laboratory equipment, open hardware, open source
SDG SDG09 Industry innovation and infrastructure
Authors Joshua M. Pearce
License CC-BY-SA-4.0
Organizations FAST, Western
Language English (en)
Translations French, Spanish, Chinese
Related 3 subpages, 34 pages link here
Impact 603 page views (more)
Created January 17, 2021 by Joshua M. Pearce
Last modified February 28, 2024 by Felipe Schenone
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