Open Source 3-D Printed ISO 8655 Compliant Multichannel Pipette

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Project data
Authors S. Chinchane
H. Kadam
K. Mowade
Joshua M. Pearce
Status Designed
Modelled
Prototyped
Verified
Verified by FAST
Links https://osf.io/9tn6e/ https://doi.org/10.5334/joh.36
Download Open Know How Manifest
Device data
Assembly instructions https://doi.org/10.5334/joh.36
Design files https://osf.io/9tn6e/
Page data
Type Project, Device
Keywords open source hardware, 3d printing, additive manufacturing, distributed manufacturing, laboratory equipment, open hardware, open source
SDG Sustainable Development Goals SDG09 Industry innovation and infrastructure
Published 2021
License CC-BY-SA-4.0
Affiliations FAST
Western
Impact Number of views to this page. Views by admins and bots are not counted. Multiple views during the same session are counted as one. 1,006
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Source: Chinchane, S., Kadam, H., Mowade, K., & Pearce, JM. 2022. Open Source 3D Printed ISO 8655 Compliant Multichannel Pipette. Journal of Open Hardware, 6(1): 1, pp. 1–18. DOI: https://doi.org/10.5334/joh.36 Academia.edu

Main design files: https://osf.io/9tn6e/ Target group: chemistry, biochemistry, biology, biomedical, medical, and associated disciplines Skills required: desktop 3D printing – easy, mechanical assembly – easy

Abstract Multichannel pipettes are used widely to accelerate research and testing in life sciences laboratories and within the biomedical industry. Commercial multichannel pipettes cost several hundred to thousand U.S. dollars and are not accessible in many areas. This study utilizes an open source and digital distributed manufacturing model to design a USD$24 4-channel 200 micro-liter pipette. The design uses widely-available interchangeable off-the-shelf parts and custom components, which can be fabricated with a low-cost fused-filament RepRap-class desktop 3D printer. The manuscript describes the design, construction, and validation of an ergonomic open-source multichannel pipette, as well as the evaluation of pipetting parameters and quality control. The open source multichannel pipette is found to be compliant with ISO 8655 standards for precision and accuracy. The device has separate syringes that operate individual tips, which prevents cross contamination of specimens because there is no direct contact of different liquids with each other and no mixing with actuating air. Each syringe is easily replaced for different experiments and the source CAD files are provided to enable others to build variants or custom configurations of the open source multichannel pipette.

Buildmultipip.png
The assembly process of the open source multichannel pipette: a) the syringes being assembled individually with springs, tips and tip holders and being inserted into the syringe holder, b) twisting motion needed to lock syringes into syringe holder, c) M3 nut and bolt are inserted into the enclosure, d) the main plunger in inserted into the enclosure, e) enclosure is tilted up so that the sliding surface that will hold the syringe holder is exposed, f) the main plunger is pushed up into the enclosure as far as possible and the syringe holder is slid into the enclosure by depressing the syringes against the springs, g) the syringe holder is pushed to the end of the slide in the enclosure, and h) the remaining enclosure component is slid into place.

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