The open-source release of self-replicating rapid prototypers (RepRaps), has created a rich opportunity for low-cost distributed digital fabrication of complex three dimensional objects such as scientific equipment. For example, 3-D printable reactionware devices offer the opportunity to combine open hardware microfluidic handling with lab on a chip reactionware to radically reduce costs and increase the number and complexity of microfluidic applications. To further drive down the cost while improving the performance of lab-on-a-chip paper-based microfluidic prototyping this study reports on the development of a RepRap upgrade capable of converting a Prusa Mendel RepRap into a wax 3-D printer for paper-based microfluidic applications. An open-source hardware approach is used to demonstrate a 3-D printable upgrade for the 3-D printer, which combines a heated syringe pump with the RepRap/Arduino 3-D control. The bill of materials, designs, basic assembly and use instructions are provided along with a completely free and open source software tool chain. The open source hardware device described here accelerates the potential of the nascent field of electrochemical detection combined with paper-based microfluidics by dropping the marginal cost of prototyping to nearly zero while accelerating the turnover between paper-based microfluidic designs.
- J. M. Pearce, N. C. Anzalone, and C. L. Heldt. Open-source Wax RepRap 3-D Printer for Rapid Prototyping Paper-Based Microfluidics, Journal of Laboratory Automation 21(4) 510–516 (2016). DOI: http://dx.doi.org/10.1177/2211068215624408 open access
- This project derived from: MOST Prusa RepRap Build it!
- STL files for 3D printing and OpenSCAD files for editing
- Open Source Software:Franklin
- Example clover print 
BOM[edit | edit source]
Assembly[edit | edit source]
Refer to paper for overall details.
- File:Prep syringe retainer.odt
- File:Prep carriage.odt
- File:Prep motor assembly.odt
- File:Prep syringe carriage.odt