TissueDB/Simulators/Intra-abdominal Bleeding Simulator (Fernandes)

The Intra-abdominal Bleeding Simulator (Fernandes) is a low-cost adult trainer built from an adult mannequin torso, silicone organs, fabric-mesh bowel and peritoneum, EVA foam, sponge and latex-tube vessels for practising control of major intra-abdominal haemorrhage during exploratory laparotomy.[1] The aorta and inferior vena cava carry simulated blood from one-litre reservoirs. Rhythmic compression of the arterial reservoir produces pulsatile flow, while the venous reservoir flows under gravity. The trainee opens the abdomen, identifies the injured vessel, controls bleeding by direct digital compression and performs hemostatic suturing.
| Field | Details |
|---|---|
| Features and Basic Operation | The model contains silicone liver, spleen, stomach, kidneys and pancreas; fabric-mesh small and large intestines; a semitransparent fabric-mesh peritoneum; an EVA-foam-and-sponge anterior access layer; and latex-tube abdominal vessels. The aorta and inferior vena cava form the active bleeding circuit. Other named abdominal vessels are positioned for anatomical realism but are not filled with fluid. The bleeding point can be changed by perforating the selected latex vessel. During the validation study, only the latex tubes and peritoneal textile mesh required replacement, twice across twelve simulations. |
| Current Development Status | Developed and content-validated by Fernandes et al. (2023) using twelve volunteer surgeons with experience in abdominal or trauma surgery. The study assessed anatomical representation, identification and access to the injured vessel, simulated blood pressure, manual compression, hemostatic suturing and the haemorrhage scenario. All questionnaire items met the study's content-validity criterion. Transfer of these skills to operating-room performance was not evaluated. |
| Estimated Build Time and Cost | - Initial construction: US$71 (2021). Maintenance during the validation study: approximately US$4.80 per simulated scenario, including minor repairs, replacement of single-use items and consumables. |
| Specialized Tools and Equipment | Commercial anatomical models of the liver, spleen, stomach, kidneys and pancreas are used as masters for the plaster moulds. A hot-glue gun is included in the source materials list and hot glue is used to fix organs in position. The simulated scenario also requires standard laparotomy instruments and appropriate lighting, which are use-time equipment rather than simulator components. |
| Version | Version 1 |
| Development Team Contact Information | Developed by Camila Oliveira Fernandes, Lucas Ribeiro Rodrigues, Mattheus Lucca Batista Silva do Amaral and Sarah Jessica de Morais Rodrigues, Centro Universitário Estácio de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil; and Marcos Antonio Marton-Filho, Universidade de São Paulo, Bauru, São Paulo, Brazil. Corresponding author: Marcos Antonio Marton-Filho (marcosmarton@gmail.com). Funded by the PIBIC institutional scholarship programme at Estácio. |
Tissues
| Tissue | Qty | Material | Cost | Notes |
|---|---|---|---|---|
| Liver | 1 | Silicone | - | Cast in silicone using a plaster mould made from a commercial anatomical model and fixed in its anatomical position. |
| Spleen | 1 | Silicone | - | Cast in silicone using a plaster mould made from a commercial anatomical model and fixed in its anatomical position. |
| Stomach | 1 | Silicone | - | Cast in silicone using a plaster mould. Positioned as a peritoneal organ after placement of the retroperitoneal structures. |
| Kidney | 2 | Silicone | - | Cast in silicone using commercial anatomical models as mould masters and positioned in the retroperitoneum. |
| Pancreas | 1 | Silicone | - | Cast in silicone using a commercial anatomical model as the mould master and positioned in the retroperitoneum. |
| Small Intestine | 1 | Fine synthetic fabric mesh + Styrofoam microspheres | - | The source uses fine synthetic fabric mesh filled with a small amount of polystyrene microspheres to retain malleability. Small and large bowel use different colours. The bowel is fixed only at its ends and no mesentery is included. |
| Large Bowel | 1 | Fine synthetic fabric mesh + Styrofoam microspheres | - | The source uses fine synthetic fabric mesh with a small amount of polystyrene microsphere filling. It is a different colour from the small intestine and is fixed only at its ends. |
| Peritoneum and Serosa | 1 | Fine semitransparent fabric mesh | - | The source describes a fine mesh of semitransparent fabric representing the peritoneal membrane and isolating the peritoneal cavity from the retroperitoneum. The TissueDB Cloth, Knitted link is the governed canonical placement; the source itself does not specify a knit construction. The separately listed waterproof fabric is not identified by the paper as this mesh. |
| Skin and Subcutaneous Tissue | 1 anterior layer | EVA foam + sponge | - | The source explicitly states that the anterior mannequin panel is replaced by an EVA plate with a thin sponge layer to mimic skin and subcutaneous tissue and permit incision and access to the abdominal cavity. |
| Aorta | 1 | Latex tube | - | Active arterial bleeding vessel. One end is occluded and the other connects to a one-litre reservoir. Rhythmic compression of the reservoir produces pulsatile flow. |
| Inferior Vena Cava | 1 | Latex tube | - | Active venous bleeding vessel. One end is occluded and the other connects to a one-litre reservoir with flow determined by gravity. |
| Other abdominal vessels | 1 set | Latex tube | - | Includes the coeliac trunk, superior and inferior mesenteric vessels, renal vessels and iliac vessels. They are positioned for anatomical realism but are not filled with simulated blood in the standard configuration. |
| Blood | Two 1-litre reservoir systems | Water with artificial red dye, or artificial blood | - | Simulated circulating fluid used in the aorta and inferior vena cava. Water remains a simulator fluid and is not a TissueDB Materials-page target. |
Structural Parts
| Part Name | Qty | Material | Cost | Notes |
|---|---|---|---|---|
| Adult mannequin torso | 1; 72 × 54 cm | Plastic | - | Provides the simulator body. The anterior section is replaced by the EVA-and-sponge access layer. The entire rear interior is covered with one EVA layer to reduce leakage during simulation. |
| Arterial reservoir | 1 litre | Saline bag | - | Feeds the aortic latex tube. Rhythmic manual compression produces pulsatile simulated arterial flow. |
| Venous reservoir | 1 litre | Serum bottle / reservoir | - | Feeds the inferior vena cava with gravity-driven flow. |
| IV infusion set | 1 set | Saline infusion kit | - | Connects the reservoirs to the active latex-tube bleeding circuit. |
Consumables
| Consumable | Quantity | Material | Approximate Cost | Notes |
|---|---|---|---|---|
| Suture thread | As needed | Surgical suture | - | Use-time consumable for hemostatic suturing during the simulated scenario. |
| Gauze | As needed | Surgical gauze | - | Use-time consumable during haemorrhage control. |
| Simulated-blood carrier fluid | Approximately 1 litre per reservoir | 0.9% saline and/or water, according to the source-described setup | - | Used as the fluid carrier for the simulated blood. Reservoirs are replenished between scenarios. |
| Red colouring | As needed | Artificial red dye / food colouring | - | Colours the carrier fluid when water-based simulated blood is used. |
Build Instructions
Phase 1: Make the silicone organs
- Obtain commercial anatomical models of the liver, spleen, stomach, kidneys and pancreas.
- Isolate each organ model and immerse it in plaster to create a negative mould.
- Allow the plaster mould to dry.
- Remove the original anatomical model from the plaster.
- Fill the resulting mould with silicone rubber.
- Allow the silicone to cure.
- Remove the silicone organ from the mould.
Editorial QA: inspect each demoulded silicone organ for gross completeness before installation. This is a TissueDB buildability check, not an author-prescribed validation criterion.
Phase 2: Prepare the mannequin and blood vessels
- Use an adult plastic mannequin torso measuring approximately 72 × 54 cm.
- Cover the entire rear interior of the mannequin with a single layer of EVA material to reduce leakage during simulation.
- Prepare latex tubing corresponding to Ref. 203, approximately 9 mm external diameter and 6 mm internal diameter.
- Position tubing to represent the aorta, inferior vena cava, coeliac trunk, superior and inferior mesenteric vessels, renal vessels and iliac vessels.
- Use the aorta and inferior vena cava as the principal active blood-circuit vessels.
- Create the required bleeding opening in the selected active vessel. In the validation scenario, the source used an approximately 2 cm partial section of the abdominal aorta at the supramesenteric level.
- Occlude one end of the aortic tube.
- Connect its other end through an infusion set to a one-litre saline bag containing simulated blood.
- Occlude one end of the inferior-vena-cava tube.
- Connect its other end to a one-litre reservoir that provides flow under gravity.
- Leave the other abdominal vessel tubes unfilled in the standard configuration.
Editorial QA: before the training scenario, confirm that rhythmic compression of the arterial reservoir produces flow from the selected arterial bleeding point and that the venous reservoir provides gravity-driven flow. This is a setup check, not a content-validation item from the paper.
Phase 3: Install the retroperitoneal organs
- Position the silicone kidneys and pancreas in the retroperitoneal region.
- Fix them in their anatomical positions using hot glue.
Phase 4: Install the peritoneal membrane
- Place a fine mesh of semitransparent fabric over the retroperitoneal structures.
- Use it to represent the peritoneal membrane separating the peritoneal cavity from the retroperitoneum.
Source limitation: the paper separately lists "waterproof fabric" in its materials-cost table but does not state that this item is the semitransparent peritoneal mesh. Do not merge those two material identities without direct evidence.
Phase 5: Install the peritoneal organs
- Position the silicone liver, spleen and stomach in their anatomical locations.
- Fix the organs using hot glue.
Phase 6: Make and position the intestines
- Use fine synthetic fabric mesh in different colours for the small and large intestines.
- Add a small amount of polystyrene microspheres to each bowel representation to preserve softness and malleability.
- Fix each intestinal representation only at its ends.
- Do not add a mesentery; the authors intentionally omitted it because it would make manipulation more difficult and increase maintenance requirements.
- Position the bowel representations in the abdominal cavity.
Phase 7: Make the anterior access layer
- Replace the anterior mannequin panel with an EVA plate measuring approximately 72 × 54 cm.
- Add a thin sponge layer to the EVA plate.
- Use this composite as the source-described skin-and-subcutaneous-tissue layer through which the laparotomy incision is made.
Editorial QA: confirm that the anterior layer provides access to the abdominal cavity without obstructing the vessel-control task. This is an editorial buildability check rather than an author-defined fabrication checkpoint.
Phase 8: Prepare the training scenario
- Provide appropriate lighting, clothing and standard instruments for exploratory laparotomy.
- Fill the active reservoir system with simulated blood.
- In the source validation scenario, create an abdominal-aortic injury with an approximately 2 cm partial section at the supramesenteric level.
- Begin the scenario with access through the anterior layer and progression to identification of the bleeding point.
- The learner performs digital compression and prepares the vessel for hemostatic suturing.
Scope and limitations
- The source configuration is an open exploratory-laparotomy simulator.
- The authors state that it could potentially be adapted to a laparoscopic training box with few modifications, but that adaptation is not the demonstrated build described here.
- The principal evaluated technical tasks were access to the vascular injury, identification of the injured vessel, direct manual compression and hemostatic suturing.
- Transfer of simulator performance to operating-room outcomes was not tested.
- The separately listed waterproof fabric has no source-defined role in the construction narrative.
| Authors | Arturopelayo |
|---|---|
| License | CC-BY-SA-4.0 |
| Cite as | Arturopelayo (2026). "TissueDB/Simulators/Intra-abdominal Bleeding Simulator (Fernandes)". Appropedia. Retrieved September 14, 2026. |
- ↑ Fernandes CO, Rodrigues LR, Silva do Amaral MLBS, de Morais Rodrigues SJ, Marton-Filho MA. "Low-cost simulator for intra-abdominal bleeding." Revista do Colégio Brasileiro de Cirurgiões. 2023;50:e20233512. DOI: 10.1590/0100-6991e-20233512-en. PMID: 37971114. PMC: PMC10618030. CC BY 4.0.