목차
Purpose목적
Glass substrates are used in many laboratory settings.유리 기판은 많은 실험실 환경에서 사용됩니다. Clean slides prevent contamination of the sample allow for correct deposition of materials.깨끗한 슬라이드는 샘플의 오염을 방지하여 재료를 올바르게 부착할 수 있도록 합니다. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface.이 방법의 주요 기능은 유리기판 표면에서 모든 것을 제거하는 전략을 제공하는 것입니다. This would effectively make the used slides function as if they were new slides.이렇게 하면 실제로 사용된 슬라이드가 새 슬라이드처럼 기능합니다.
Methodology방법론
Method 1 (For cleaned substrates with higher tolerances)
Acetone
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Propan-2-ol
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Deionized Water
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Each solution can be made into a heated bath to improve the cleaning effect.
Method 2 (For cleaned substrates with tighter tolerances)
Alkaline Solution(dawn dish soap)
Apply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution).
Acetone [1]
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Methanolic HCl [2]
Dip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas.
Oxygen Etching
Follow proper protocol for device used.
Propan-2-ol [3]
Dip slide in 10% KOH in isopropanol for 30 minutes,rinse in DI water, dry under Nitrogen gas.
Sodium Hydroxide [4] (Acid Bath)
Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in Piranha solution (Optional),rinse in DI water, dry under Nitrogen gas.
Applications적용들
- General substrate control.
- Micro-scale cleaning
Cost비용.
| Method | Cost of Solution (Per Litre) | Cost/Batch (30 Substrates dipped in 200ml of Solution) |
|---|---|---|
| Acetone | $25-40 | $5-8 |
| Propanol | $20-40 | $4-8 |
| Dawn dish soap | $10-15 | $2-3 |
| Methanolic HCl | $40-80 (mixture of Hcl and MeOH) | $8-16 (100ml MeOH + 100ml HCl) |
| Propanol | $28-40 | $5.6-8 |
| Sodium Hydroxide | $18-52 | $3.6-10.4 |
References레퍼런스
- ↑ UC Irvine. "Cleaning procedures for glass substrates," PDF
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999