Glass Substrate Cleaning
Purpose[edit | edit source]
Glass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides.
Methodology[edit | edit source]
Method 1 (For cleaned substrates with higher tolerances)
Acetone[edit | edit source]
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Propan-2-ol[edit | edit source]
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Deionized Water[edit | edit source]
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Each solution can be made into a heated bath to improve the cleaning effect.
Method 2 (For cleaned substrates with tighter tolerances)
Alkaline Solution(dawn dish soap)[edit | edit source]
Apply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution).
Acetone[1][edit | edit source]
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Methanolic HCl[2][edit | edit source]
Dip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas.
Oxygen Etching[edit | edit source]
Follow proper protocol for device used.
Propan-2-ol[3][edit | edit source]
Dip slide in 10% KOH in isopropanol for 30 minutes, rinse in DI water, dry under Nitrogen gas.
Sodium Hydroxide[4] (Acid Bath)[edit | edit source]
Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in Piranha solution (Optional), rinse in DI water, dry under Nitrogen gas.
Applications[edit | edit source]
- General substrate control.
- Micro-scale cleaning
Cost[edit | edit source]
| Method | Cost of Solution (Per Litre) | Cost/Batch (30 Substrates dipped in 200ml of Solution) |
|---|---|---|
| Acetone | $25-40 | $5-8 |
| Propanol | $20-40 | $4-8 |
| Dawn dish soap | $10-15 | $2-3 |
| Methanolic HCl | $40-80 (mixture of Hcl and MeOH) | $8-16 (100ml MeOH + 100ml HCl) |
| Propanol | $28-40 | $5.6-8 |
| Sodium Hydroxide | $18-52 | $3.6-10.4 |
References[edit | edit source]
- ↑ UC Irvine. "Cleaning procedures for glass substrates," PDF
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
