Glass substrate cleaning method: MOST
Glass Substrate Cleaning
Purpose
[edit | edit source]Glass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides.
Methodology
[edit | edit source]Method 1 (For cleaned substrates with higher tolerances)
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Each solution can be made into a heated bath to improve the cleaning effect.
Method 2 (For cleaned substrates with tighter tolerances)
Apply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution).
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Dip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas.
Follow proper protocol for device used.
Dip slide in 10% KOH in isopropanol for 30 minutes, rinse in DI water, dry under Nitrogen gas.
Sodium Hydroxide[4] (Acid Bath)
[edit | edit source]Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in Piranha solution (Optional), rinse in DI water, dry under Nitrogen gas.
Applications
[edit | edit source]- General substrate control.
- Micro-scale cleaning
Cost
[edit | edit source]| Method | Cost of Solution (Per Litre) | Cost/Batch (30 Substrates dipped in 200ml of Solution) |
|---|---|---|
| Acetone | $25-40 | $5-8 |
| Propanol | $20-40 | $4-8 |
| Dawn dish soap | $10-15 | $2-3 |
| Methanolic HCl | $40-80 (mixture of Hcl and MeOH) | $8-16 (100ml MeOH + 100ml HCl) |
| Propanol | $28-40 | $5.6-8 |
| Sodium Hydroxide | $18-52 | $3.6-10.4 |
References
[edit | edit source]- ↑ UC Irvine. "Cleaning procedures for glass substrates," PDF
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
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| License | CC-BY-SA-3.0 |
| Cite as | Nyamoga, Ampringl (2016–2025). "Glass substrate cleaning method: MOST". Appropedia. Retrieved June 21, 2026. |