{"id":68205,"key":"Glass_substrate_cleaning_method:_MOST","title":"Glass substrate cleaning method: MOST","latest":{"id":1208746,"timestamp":"2025-11-28T00:46:25Z"},"content_model":"wikitext","license":{"url":"https://www.appropedia.org/Appropedia:Copyrights","title":"CC-BY-SA-4.0"},"source":"{{MOST}}\n\n '''Glass Substrate Cleaning'''\n\n== Purpose ==\n\nGlass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides.\n\n== Methodology ==\n\n Method 1 (For cleaned substrates with higher tolerances)\n\n=== [https://www.inrf.uci.edu/wordpress/wp-content/uploads/sop-wet-cleaning-pro-for-glass-substrates.pdf Acetone] ===\n\nPour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).\n\nPlace glass substrate in warm Acetone for 10 minutes.\n\nPlace glass substrate in methanol for 2-5 minutes.\n\nRemove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).\n\n=== [https://en.wikipedia.org/wiki/Isopropyl_alcohol Propan-2-ol] ===\n\nApply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).\n\n=== [http://www.conservation-wiki.com/wiki/Deionized_water Deionized Water] ===\n\nApply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).\n\nEach solution can be made into a heated bath to improve the cleaning effect.\n\n Method 2 (For cleaned substrates with tighter tolerances)\n\n=== [https://en.wikipedia.org/wiki/Alkali Alkaline Solution(dawn dish soap)] ===\n\nApply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution).\n\n=== [https://www.inrf.uci.edu/wordpress/wp-content/uploads/sop-wet-cleaning-pro-for-glass-substrates.pdf Acetone]<ref>UC Irvine. \"Cleaning procedures for glass substrates,\" PDF</ref> ===\n\nPour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).\n\nPlace glass substrate in warm Acetone for 10 minutes.\n\nPlace glass substrate in methanol for 2-5 minutes.\n\nRemove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).\n\n=== [http://www.sciencedirect.com/science/article/pii/S0956566399000433 Methanolic HCl]<ref>J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. \"Comparison of chemical cleaning methods of glass in preparation for salinization,\" Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999</ref> ===\n\nDip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas.\n\n=== [https://en.wikipedia.org/wiki/Plasma_etching Oxygen Etching] ===\n\nFollow proper protocol for device used.\n\n=== [http://www.sciencedirect.com/science/article/pii/S0956566399000433 Propan-2-ol]<ref>J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. \"Comparison of chemical cleaning methods of glass in preparation for salinization,\" Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999</ref> ===\n\nDip slide in 10% KOH in isopropanol for 30 minutes,\nrinse in DI water, dry under Nitrogen gas.\n\n=== [http://www.sciencedirect.com/science/article/pii/S0956566399000433 Sodium Hydroxide<ref>J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. \"Comparison of chemical cleaning methods of glass in preparation for salinization,\" Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999</ref> ] (Acid Bath) ===\n\nDip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in [https://en.wikipedia.org/wiki/Piranha_solution Piranha solution] (Optional),\nrinse in DI water, dry under Nitrogen gas.\n\n== Applications ==\n\n* General substrate control.\n* Micro-scale cleaning\n\n== Cost ==\n\n{| class=\"wikitable\" style=\"float:right; margin:.5em\"\n! Method\n! Cost of Solution (Per Litre)\n! Cost/Batch (30 Substrates dipped in 200ml of Solution)\n|-\n| Acetone\n| $25-40\n| $5-8\n|-\n| Propanol\n| $20-40\n| $4-8\n|-\n| Dawn dish soap\n| $10-15\n| $2-3\n|-\n| Methanolic HCl\n| $40-80 (mixture of Hcl and MeOH)\n| $8-16 (100ml MeOH + 100ml HCl)\n|-\n| Propanol\n| $28-40\n| $5.6-8\n|-\n| Sodium Hydroxide\n| $18-52\n| $3.6-10.4\n|}\n\n{{clear}}\n\n== References ==\n\n<references />\n\n{{Page data\n| license = CC-BY-SA-3.0\n}}\n\n[[Category:MOST methods]]"}