Dr. Frost UV spectroscopy unit protocol MOST
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Gloves and safety glasses[edit | edit source]
Dr. Frost requires everyone who is working in her lab wear gloves and safety glasses.
Lamps[edit | edit source]
In order to switch the lamps on, you must flip the green switch to on.
- Click start button
- Wait at least ten minutes for the machine to set up before starting program
- There should be a loud cranking noise, which means that the machine is working correctly.
- The icon you want is called Scan-Lambda 35
Cuvettes[edit | edit source]
The reference cuvette is the one that is farthest away from the front of the machine.
- Located near the back of the UV chamber.
A beam of light will be split into two beams
- These beams will shine through your sample and a reference then compare the two wavelengths
The sample cuvette should be placed in the sample cuvette holder
- The sample should be taped as flat as possible against the cuvette's outer layer
- do not tape sample to the inside of the cuvette
Once you have the cuvettes and your samples prepared, autozero and start the machine.
Export[edit | edit source]
To export click file->export, then pick a destination file to export.
The most important detail about exporting your data is to switch the Data from "spectrum export file" to "XY Data (raw)"
Finding the spectroscopy beam[edit | edit source]
In order to find out exactly where the UV spectrometer is shining its light, you have to set it to the green wavelength (anywhere from 577-492 nm). First click the change lambda button, then enter your desired wavelength into the input box, and finally use a slip of paper with the UV spectrometer open to find the few-nanometer-wide slit.