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Dr. Frost UV spectroscopy unit protocol MOST

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Gloves and safety glasses[edit]

Dr. Frost requires everyone who is working in her lab wear gloves and safety glasses.


Lamps[edit]

In order to switch the lamps on, you must flip the green switch to on.

  • Click start button
  • Wait at least ten minutes for the machine to set up before starting program
  • There should be a loud cranking noise, which means that the machine is working correctly.

program details:

  • The icon you want is called Scan-Lambda 35

Cuvettes[edit]

The reference cuvette is the one that is farthest away from the front of the machine.

  • Located near the back of the UV chamber.

A beam of light will be split into two beams

  • These beams will shine through your sample and a reference then compare the two wavelengths

The sample cuvette should be placed in the sample cuvette holder

  • The sample should be taped as flat as possible against the cuvette's outer layer
    • do not tape sample to the inside of the cuvette

Once you have the cuvettes and your samples prepared, autozero and start the machine.

Export[edit]

To export click file->export, then pick a destination file to export.

The most important detail about exporting your data is to switch the Data from "spectrum export file" to "XY Data (raw)"


Finding the spectroscopy beam[edit]

In order to find out exactly where the UV spectrometer is shining its light, you have to set it to the green wavelength (anywhere from 577-492 nm). First click the change lambda button, then enter your desired wavelength into the input box, and finally use a slip of paper with the UV spectrometer open to find the few-nanometer-wide slit.