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Gloves and safety glasses[edit | edit source]

Dr. Frost requires everyone who is working in her lab wear gloves and safety glasses.

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Lamps[edit | edit source]

In order to switch the lamps on, you must flip the green switch to on.

• Click start button
• Wait at least ten minutes for the machine to set up before starting program
• There should be a loud cranking noise, which means that the machine is working correctly.

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  UV spectroscopy unit switched to on

program details:

• The icon you want is called Scan-Lambda 35

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  program icon

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  when the program is first started, it will initialize

Cuvettes[edit | edit source]

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  Reference is to the right, samples to the left

The reference cuvette is the one that is farthest away from the front of the machine.

• Located near the back of the UV chamber.

A beam of light will be split into two beams

• These beams will shine through your sample and a reference then compare the two wavelengths

The sample cuvette should be placed in the sample cuvette holder

• The sample should be taped as flat as possible against the cuvette's outer layer
  • do not tape sample to the inside of the cuvette

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  Samples attached with tape to cuvette

Once you have the cuvettes and your samples prepared, autozero and start the machine.

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  autozero the machine

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  click "OK" to autozero

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  click start button to start running samples

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  click "OK"

Export[edit | edit source]

To export click file->export, then pick a destination file to export.

The most important detail about exporting your data is to switch the Data from "spectrum export file" to "XY Data (raw)"

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  file->export

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  browse for destination folder

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  select which folder you want

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  switch checked boxes

Finding the spectroscopy beam[edit | edit source]

In order to find out exactly where the UV spectrometer is shining its light, you have to set it to the green wavelength (anywhere from 577-492 nm). First click the change lambda button, then enter your desired wavelength into the input box, and finally use a slip of paper with the UV spectrometer open to find the few-nanometer-wide slit.

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  click the set lamda button

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  insert slip of paper with the overhead lights turned off.