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{{reflist}} | {{reflist}} | ||
=== | ===Cuvettes=== | ||
The reference | The reference cuvette is the one that is farthest away from the front of the machine. | ||
*Located near the back of the UV chamber. | *Located near the back of the UV chamber. | ||
A beam of light will be split into two beams | A beam of light will be split into two beams | ||
*These beams will shine through your sample and a reference then compare the two wavelengths | *These beams will shine through your sample and a reference then compare the two wavelengths | ||
The sample | The sample cuvette should be placed in the sample cuvette holder | ||
*The sample should be taped as flat as possible against the | *The sample should be taped as flat as possible against the cuvette's outer layer | ||
**do not tape sample to the inside of the | **do not tape sample to the inside of the cuvette | ||
== Idle == | == Idle == | ||
Revision as of 16:28, 6 June 2014
Lamps
For now you must turn the lamps on manually on the computer by checking the two boxes that say "UV Lamp"
- Click start button
- Wait for the machine to set up
Cuvettes
The reference cuvette is the one that is farthest away from the front of the machine.
- Located near the back of the UV chamber.
A beam of light will be split into two beams
- These beams will shine through your sample and a reference then compare the two wavelengths
The sample cuvette should be placed in the sample cuvette holder
- The sample should be taped as flat as possible against the cuvette's outer layer
- do not tape sample to the inside of the cuvette
Idle
1. Auto zero the machine by pressing autozero
2. Put samples into respective places
3. Click the start button
Export
XY data by column
Raw spectrum file