J.M.Pearce (talk | contribs) (Created page with "{{MOST}} category:MOST methods This page gives instructions on how to use your cell phone to take contact angle measurements. ==Experimental Instructions== # Put your sa...") |
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# Open imageJ and check to see if "Contant Angle" is available under the "Plugins" tab and select it. | # Open imageJ and check to see if "Contant Angle" is available under the "Plugins" tab and select it. | ||
# Open the image. | # Open the image. | ||
# Select the two end points of the droplet where it meets the substrate using the "drop points" button. | # Select the two end points of the droplet where it meets the substrate using the "drop points" button (shown in red). | ||
# Select 5 points along the droplet surface and allow imageJ to run the fitting algorithm. | # Select 5 points along the droplet surface and allow imageJ to run the fitting algorithm (shown in blue). | ||
# Click on the Menu button in the Contact Angle plugin toolbar. | # Click on the Menu button in the Contact Angle plugin toolbar. | ||
# Click Manual Points Procedure. | # Click Manual Points Procedure. | ||
# Open the results window to see the contact angle for a spherical approximation and also for elliptical approximation. | # Open the results window to see the contact angle for a spherical approximation and also for elliptical approximation. | ||
[[File:CellphoneCA.PNG]] | |||
==Drawbacks== | ==Drawbacks== | ||
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# Uses either a spherical or an elliptical approximation for the droplet. The user needs to be able to pick manually which approximation works better for their case. | # Uses either a spherical or an elliptical approximation for the droplet. The user needs to be able to pick manually which approximation works better for their case. | ||
# The drop has to be very very small (ideally <3ul). The program neglects gravity. | # The drop has to be very very small (ideally <3ul). The program neglects gravity. | ||
==For more information== | |||
1. [https://www.researchgate.net/publication/328733959_Measuring_the_Contact_Angle_using_ImageJ_with_Contact_Angle_plug-in ResearchGate example] | |||
2. [https://www.youtube.com/watch?v=eLzcRvFneTM Youtube tutorial] |
Revision as of 15:48, 12 December 2019
This page gives instructions on how to use your cell phone to take contact angle measurements.
Experimental Instructions
- Put your sample on a flat substrate
- Use a micropipette to deposit a small droplet of fluid on the sample
- Take a picture of the droplet from the side of the substrate as shown in Figure below. Make sure the image is in focus and not blurry.
Software analysis
- Install the open source imageJ plugin for contact angle measurements: https://imagej.nih.gov/ij/plugins/contact-angle.html
- Open imageJ and check to see if "Contant Angle" is available under the "Plugins" tab and select it.
- Open the image.
- Select the two end points of the droplet where it meets the substrate using the "drop points" button (shown in red).
- Select 5 points along the droplet surface and allow imageJ to run the fitting algorithm (shown in blue).
- Click on the Menu button in the Contact Angle plugin toolbar.
- Click Manual Points Procedure.
- Open the results window to see the contact angle for a spherical approximation and also for elliptical approximation.
Drawbacks
- This will not work if the substrate is not absolutely flat. The program needs to be able to draw a straight line that follows the surface of the substrate exactly after the user selects the two end points of the droplet.
- Uses either a spherical or an elliptical approximation for the droplet. The user needs to be able to pick manually which approximation works better for their case.
- The drop has to be very very small (ideally <3ul). The program neglects gravity.