Particle Size Analysis using ImageJ Software: Difference between revisions

Setup

Helpful video (Made by someone else): https://www.youtube.com/watch?v=JQAZ--c9YfI

1. Items Required: A. Particles B. Ruler (or Scale on Image) C. Camera (Cellphone or Digital) that can take high quality photos D. High Contrast Background, either white or black depending on particle color

2. Installing FIJI (Fiji Is Just ImageJ): Download link: https://fiji.sc/ Taking Photos: Place the particles and a ruler on a plain high contrast background. Make sure the particles are not touching each other, you will get bad results. Make sure the light is soft, and there are no shadows being created by the particles. Here is a good example:

ImageJ 3. Open the ImageJ Software 4. Open image E. File > Open > Select Image 5. Save as something memorable F. File > Save as > “PLA Pellet Distribution 6-1-2018” 6. Set Scale on Image G. Select Line Tool H. Hold control and scroll to zoom I. Draw line on ruler a set distance (ex. From 50 to 60mm) – Click and Drag

J. Go to Analyze > Set Scale K. Set known distance and units (ex. 10mm)

L. Select OK, and your image in now calibrated to scale.

7. Draw Rectangle around particles

8. Duplicate image by going to Image > Duplicate. It will open a new window with just the particles in the image.

9. Set Image as 8 bit by going Image > Type > 8 Bit

10. Set bandpass filter by going Process > FFT > Bandpass Filter. Hit OK without changing any settings.

11. You are now ready to perform a particle distribution; the previous steps were to get the image ready for analysis. 12. Adjust the threshold by going to Image > Adjust > Threshold 13. Move the two bars until you get black where the particles are, and a white background, it is ok if there is specks still showing, we will ignore those in the next steps. Example shown:

14. Hit apply 15. Analyze the particle size by going Analyze > Analyze Particles 16. Set size to about the size you would expect, this can be tweaked to make sure that the specks are not being counted. You can also tweak the circularity of the particles as well. Go to Show > Outlines to output a picture of what the software is measuring 17. Check: Display Results and Include holes

18. Results are shown in a new window, as well as the image showing the particles that are actually being measured. Make sure that particles are not being counted as two together, this will skew area results.

19. The results window can be saved to a text document, where they can be formatted and analyzed further. 20. Done!

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