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===[https://en.wikipedia.org/wiki/Alkali alkaline solution(dawn dish soap)]=== | ===[https://en.wikipedia.org/wiki/Alkali alkaline solution(dawn dish soap)]=== | ||
Apply to slide, rub with paper towel and rinse off. (if possible ultrasonically clean in solution). | Apply to slide, rub with paper towel and rinse off. (if possible ultrasonically clean in solution). | ||
===[https://en.wikipedia.org/wiki/Acetone acetone]=== | ===[https://en.wikipedia.org/wiki/Acetone acetone] <ref> UC Irvine "Cleaning procedures for glass substrates," PDF </ref>=== | ||
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
===[https://en.wikipedia.org/wiki/Isopropyl_alcohol propan-2-ol]=== | ===[https://en.wikipedia.org/wiki/Isopropyl_alcohol propan-2-ol]=== | ||
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
Revision as of 17:25, 14 July 2016
Glass Substrate Cleaning
Purpose
Glass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides.
Methodology
Method 1 (For cleaned substrates with higher tolerances)
=acetone
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C)
Place glass substrate in warm Acetone for 10 minutes
Place glass substrate in methanol for 2-5 minutes
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution)
propan-2-ol
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Deionized Water
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Each solution can be made into a heated bath to improve the cleaning effect.
Method 2 (For cleaned substrates with tighter tolerances)
alkaline solution(dawn dish soap)
Apply to slide, rub with paper towel and rinse off. (if possible ultrasonically clean in solution).
acetone [1]
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
propan-2-ol
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
acid bath(Piranha)
Dip slide into a hot bath 3:1 ratio of sulfuric acid to 30% hydrogen peroxide solution
oxygen etching
Follow proper protocol for device used
Applications
- General substrate control.
- Micro-scale cleaning
References
- ↑ UC Irvine "Cleaning procedures for glass substrates," PDF