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Dr. Frost UV spectroscopy unit protocol MOST

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Lamps

In order to switch the lamps on, you must flip the green switch to on.

  • Click start button
  • Wait for the machine to set up

Cuvettes

The reference cuvette is the one that is farthest away from the front of the machine.

  • Located near the back of the UV chamber.

A beam of light will be split into two beams

  • These beams will shine through your sample and a reference then compare the two wavelengths

The sample cuvette should be placed in the sample cuvette holder

  • The sample should be taped as flat as possible against the cuvette's outer layer
    • do not tape sample to the inside of the cuvette

Idle

1. Auto zero the machine by pressing autozero

2. Put samples into respective places

3. Click the start button

Export

XY data by column

Raw spectrum file