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The reference cubette is the one that is farthest away from the front of the machine. | The reference cubette is the one that is farthest away from the front of the machine. | ||
*Located near the back of the UV chamber. | *Located near the back of the UV chamber. | ||
A beam of light will be split into two beams | |||
*These beams will shine through your sample and a reference then compare the two wavelengths | |||
The sample cubette should be placed in the sample cubette holder | The sample cubette should be placed in the sample cubette holder | ||
*The sample should be taped as flat as possible against the cubette's outer layer | *The sample should be taped as flat as possible against the cubette's outer layer | ||
Revision as of 16:02, 6 June 2014
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Lamps
For now you must turn the lamps on manually on the computer by checking the two boxes that say "UV Lamp"
- Click start button
- Wait for the machine to set up
Cubettes
The reference cubette is the one that is farthest away from the front of the machine.
- Located near the back of the UV chamber.
A beam of light will be split into two beams
- These beams will shine through your sample and a reference then compare the two wavelengths
The sample cubette should be placed in the sample cubette holder
- The sample should be taped as flat as possible against the cubette's outer layer
- do not tape sample to the inside of the cubette
Idle
1. Auto zero the machine by pressing autozero
2. Put samples into respective places
3. Hit start
Export
XY data by column
Raw spectrum file