Dr. Frost UV spectroscopy unit protocol MOST: Difference between revisions

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Lamps

For now you must turn the lamps on manually on the computer by checking the two boxes that say "UV Lamp"

• Click start button
• Wait for the machine to set up

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Cubettes

The reference cubette is the one that is farthest away from the front of the machine.

• Located near the back of the UV chamber.
• A beam of light will be split into two beams
  • These beams will shine through your sample and a reference then compare the two wavelengths

The sample cubette should be placed in the sample cubette holder

• The sample should be taped as flat as possible against the cubette's outer layer
  • do not tape sample to the inside of the cubette
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Idle

1. Auto zero the machine by pressing autozero

2. Put samples into respective places

3. Hit start

Export

XY data by column

Raw spectrum file