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==Methodology== | ==Methodology== | ||
Method 1 (For cleaned substrates with higher tolerances) | Method 1 (For cleaned substrates with higher tolerances) | ||
===[https:// | ===[https://www.inrf.uci.edu/wordpress/wp-content/uploads/sop-wet-cleaning-pro-for-glass-substrates.pdf Acetone]=== | ||
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C) | Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C). | ||
Place glass substrate in warm Acetone for 10 minutes | Place glass substrate in warm Acetone for 10 minutes. | ||
Place glass substrate in methanol for 2-5 minutes | Place glass substrate in methanol for 2-5 minutes. | ||
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution) | Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
===[https://en.wikipedia.org/wiki/Isopropyl_alcohol | ===[https://en.wikipedia.org/wiki/Isopropyl_alcohol Propan-2-ol]=== | ||
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
===[http://www.conservation-wiki.com/wiki/Deionized_water Deionized Water]=== | ===[http://www.conservation-wiki.com/wiki/Deionized_water Deionized Water]=== | ||
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
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Method 2 (For cleaned substrates with tighter tolerances) | Method 2 (For cleaned substrates with tighter tolerances) | ||
===[https://en.wikipedia.org/wiki/Alkali | ===[https://en.wikipedia.org/wiki/Alkali Alkaline Solution(dawn dish soap)]=== | ||
Apply to slide, rub with paper towel and rinse off | Apply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution). | ||
===[https:// | |||
===[https://www.inrf.uci.edu/wordpress/wp-content/uploads/sop-wet-cleaning-pro-for-glass-substrates.pdf Acetone] <ref> UC Irvine. "Cleaning procedures for glass substrates," PDF </ref>=== | |||
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C) | Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C). | ||
Place glass substrate in warm Acetone for 10 minutes | Place glass substrate in warm Acetone for 10 minutes. | ||
Place glass substrate in methanol for 2-5 minutes | Place glass substrate in methanol for 2-5 minutes. | ||
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution) | Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
===[ | ===[http://www.sciencedirect.com/science/article/pii/S0956566399000433 Methanolic HCl] <ref> J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999 </ref>=== | ||
Dip slide in 1:1 MeOH: | Dip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas. | ||
===[https://en.wikipedia.org/wiki/Plasma_etching | ===[https://en.wikipedia.org/wiki/Plasma_etching Oxygen Etching]=== | ||
Follow proper protocol for device used | Follow proper protocol for device used. | ||
===[ | ===[http://www.sciencedirect.com/science/article/pii/S0956566399000433 Propan-2-ol] <ref> J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999 </ref> === | ||
Dip slide in 10% KOH in isopropanol for 30 minutes, | Dip slide in 10% KOH in isopropanol for 30 minutes, | ||
rinse in DI water, dry under Nitrogen gas. | |||
===[ | ===[http://www.sciencedirect.com/science/article/pii/S0956566399000433 Sodium Hydroxide <ref> J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999 </ref> ] (Acid Bath)=== | ||
Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in [https://en.wikipedia.org/wiki/Piranha_solution Piranha solution] (Optional), | Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in [https://en.wikipedia.org/wiki/Piranha_solution Piranha solution] (Optional), | ||
rinse in DI water, dry under Nitrogen gas. | |||
==Applications== | ==Applications== | ||
Revision as of 19:01, 23 July 2016
Glass Substrate Cleaning
Purpose
Glass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides.
Methodology
Method 1 (For cleaned substrates with higher tolerances)
Acetone
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Propan-2-ol
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Deionized Water
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Each solution can be made into a heated bath to improve the cleaning effect.
Method 2 (For cleaned substrates with tighter tolerances)
Alkaline Solution(dawn dish soap)
Apply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution).
Acetone [1]
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Methanolic HCl [2]
Dip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas.
Oxygen Etching
Follow proper protocol for device used.
Propan-2-ol [3]
Dip slide in 10% KOH in isopropanol for 30 minutes, rinse in DI water, dry under Nitrogen gas.
Sodium Hydroxide [4] (Acid Bath)
Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in Piranha solution (Optional), rinse in DI water, dry under Nitrogen gas.
Applications
- General substrate control.
- Micro-scale cleaning
Cost
| Method | Cost of Solution (Per Litre) | Cost/Batch (30 Substrates dipped in 200ml of Solution) |
|---|---|---|
| Acetone | $25-40 | $5-8 |
| Propanol | $20-40 | $4-8 |
| Dawn dish soap | $10-15 | $2-3 |
| Methanolic HCl | $40-80 (mixture of Hcl and MeOH) | $8-16 (100ml MeOH + 100ml HCl) |
| Propanol | $28-40 | $5.6-8 |
| Sodium Hydroxide | $18-52 | $3.6-10.4 |
References
- ↑ UC Irvine. "Cleaning procedures for glass substrates," PDF
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999