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'''Glass Substrate Cleaning''' | |||
Glass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides. | == Purpose == | ||
Glass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides. | |||
== Methodology == | |||
Method 1 (For cleaned substrates with higher tolerances) | Method 1 (For cleaned substrates with higher tolerances) | ||
===[https:// | |||
=== [https://www.inrf.uci.edu/wordpress/wp-content/uploads/sop-wet-cleaning-pro-for-glass-substrates.pdf Acetone] === | |||
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C). | Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C). | ||
| Line 17: | Line 21: | ||
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution). | Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
===[https://en.wikipedia.org/wiki/Isopropyl_alcohol Propan-2-ol]=== | === [https://en.wikipedia.org/wiki/Isopropyl_alcohol Propan-2-ol] === | ||
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
===[http://www.conservation-wiki.com/wiki/Deionized_water Deionized Water]=== | === [http://www.conservation-wiki.com/wiki/Deionized_water Deionized Water] === | ||
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution). | ||
Each solution can be made into a heated bath to improve the cleaning effect. | Each solution can be made into a heated bath to improve the cleaning effect. | ||
Method 2 (For cleaned substrates with tighter tolerances) | |||
=== [https://en.wikipedia.org/wiki/Alkali Alkaline Solution(dawn dish soap)] === | |||
Apply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution). | |||
=== [https://www.inrf.uci.edu/wordpress/wp-content/uploads/sop-wet-cleaning-pro-for-glass-substrates.pdf Acetone]<ref>UC Irvine. "Cleaning procedures for glass substrates," PDF</ref> === | |||
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C). | |||
Place glass substrate in warm Acetone for 10 minutes. | |||
Place glass substrate in methanol for 2-5 minutes. | |||
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution). | |||
=== [http://www.sciencedirect.com/science/article/pii/S0956566399000433 Methanolic HCl]<ref>J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999</ref> === | |||
Dip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas. | |||
=== [https://en.wikipedia.org/wiki/Plasma_etching Oxygen Etching] === | |||
Follow proper protocol for device used. | |||
===[ | === [http://www.sciencedirect.com/science/article/pii/S0956566399000433 Propan-2-ol]<ref>J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999</ref> === | ||
Dip slide in 10% KOH in isopropanol for 30 minutes, | Dip slide in 10% KOH in isopropanol for 30 minutes, | ||
rinse in DI water, dry under Nitrogen gas. | |||
=== [http://www.sciencedirect.com/science/article/pii/S0956566399000433 Sodium Hydroxide<ref>J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999</ref> ] (Acid Bath) === | |||
Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in [https://en.wikipedia.org/wiki/Piranha_solution Piranha solution] (Optional), | Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in [https://en.wikipedia.org/wiki/Piranha_solution Piranha solution] (Optional), | ||
rinse in DI water, dry under Nitrogen gas. | |||
==Applications== | == Applications == | ||
*General substrate control. | |||
*Micro-scale cleaning | * General substrate control. | ||
* Micro-scale cleaning | |||
== Cost == | |||
{| class="wikitable" style="float:right; margin:.5em" | {| class="wikitable" style="float:right; margin:.5em" | ||
! Method | |||
! Method | ! Cost of Solution (Per Litre) | ||
! Cost of Solution (Per Litre) | |||
! Cost/Batch (30 Substrates dipped in 200ml of Solution) | ! Cost/Batch (30 Substrates dipped in 200ml of Solution) | ||
|- | |- | ||
| Line 69: | Line 82: | ||
|- | |- | ||
| Propanol | | Propanol | ||
| $20-40 | | $20-40 | ||
| $4-8 | | $4-8 | ||
|- | |- | ||
| Dawn dish soap | | Dawn dish soap | ||
| $10-15 | | $10-15 | ||
| $2-3 | | $2-3 | ||
|- | |- | ||
| Line 80: | Line 93: | ||
| $8-16 (100ml MeOH + 100ml HCl) | | $8-16 (100ml MeOH + 100ml HCl) | ||
|- | |- | ||
| Propanol | | Propanol | ||
| $28-40 | | $28-40 | ||
| $5.6-8 | | $5.6-8 | ||
|- | |- | ||
|Sodium Hydroxide | | Sodium Hydroxide | ||
|$18-52 | | $18-52 | ||
|$3.6-10.4 | | $3.6-10.4 | ||
|} | |} | ||
{{clear}} | {{clear}} | ||
==References== | == References == | ||
<references /> | |||
{{Page data}} | |||
[[Category:MOST methods]] | |||
Latest revision as of 12:56, 9 June 2023
Glass Substrate Cleaning
Purpose[edit | edit source]
Glass substrates are used in many laboratory settings. Clean slides prevent contamination of the sample allow for correct deposition of materials. The primary function of this method is to provide a strategy to remove everything from a glass substrate surface. This would effectively make the used slides function as if they were new slides.
Methodology[edit | edit source]
Method 1 (For cleaned substrates with higher tolerances)
Acetone[edit | edit source]
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Propan-2-ol[edit | edit source]
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Deionized Water[edit | edit source]
Apply to slide, dry with nitrogen gas (if possible ultrasonically clean in solution).
Each solution can be made into a heated bath to improve the cleaning effect.
Method 2 (For cleaned substrates with tighter tolerances)
Alkaline Solution(dawn dish soap)[edit | edit source]
Apply to slide, rub with paper towel and rinse off (if possible ultrasonically clean in solution).
Acetone[1][edit | edit source]
Pour Acetone in a container and warm it up on a hot plate (not exceeding 55 deg C).
Place glass substrate in warm Acetone for 10 minutes.
Place glass substrate in methanol for 2-5 minutes.
Remove and rinse in DI water, then blow dry with nitrogen gas (if possible ultrasonically clean in solution).
Methanolic HCl[2][edit | edit source]
Dip slide in 1:1 MeOH:HCl for 30 minutes, then rinse in DI water, and dry under Nitrogen gas.
Oxygen Etching[edit | edit source]
Follow proper protocol for device used.
Propan-2-ol[3][edit | edit source]
Dip slide in 10% KOH in isopropanol for 30 minutes, rinse in DI water, dry under Nitrogen gas.
Sodium Hydroxide[4] (Acid Bath)[edit | edit source]
Dip slide for 20 minutes in 1M NaOH solution, incubate for 30 minutes in Piranha solution (Optional), rinse in DI water, dry under Nitrogen gas.
Applications[edit | edit source]
- General substrate control.
- Micro-scale cleaning
Cost[edit | edit source]
| Method | Cost of Solution (Per Litre) | Cost/Batch (30 Substrates dipped in 200ml of Solution) |
|---|---|---|
| Acetone | $25-40 | $5-8 |
| Propanol | $20-40 | $4-8 |
| Dawn dish soap | $10-15 | $2-3 |
| Methanolic HCl | $40-80 (mixture of Hcl and MeOH) | $8-16 (100ml MeOH + 100ml HCl) |
| Propanol | $28-40 | $5.6-8 |
| Sodium Hydroxide | $18-52 | $3.6-10.4 |
References[edit | edit source]
- ↑ UC Irvine. "Cleaning procedures for glass substrates," PDF
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
- ↑ J.J Cras, C.A Rowe-Taitt, D.A Nivens, F.S Ligler. "Comparison of chemical cleaning methods of glass in preparation for salinization," Biosensors and Bioelectronics, vol.14, No. 8-9, pp.683-688, Dec 1999
